Troubleshooting StatusCode.PERMISSION_DENIED

Help & Support
1

I’m trying to follow the quickstart tutorial using a jupyter notebook hosted on the All of Us research platform.

I’ve created an API key.

The following code executes without errors:

from alphagenome import colab_utils
from alphagenome.data import gene_annotation
from alphagenome.data import genome
from alphagenome.data import transcript as transcript_utils
from alphagenome.interpretation import ism
from alphagenome.models import dna_client
from alphagenome.models import variant_scorers
from alphagenome.visualization import plot_components

dna_model = dna_client.create('MY_SECRET_KEY_REDACTED')
variant = genome.Variant(
    chromosome='chr22',
    position=36201698,
    reference_bases='A',
    alternate_bases='C',
)
interval = variant.reference_interval.resize(dna_client.SEQUENCE_LENGTH_1MB)

However, when I try to use the predict_variant function, I get a 403 error.

Code:

outputs = dna_model.predict_variant(
    interval=interval,
    variant=variant,
    ontology_terms=['UBERON:0001157'],
    requested_outputs=[dna_client.OutputType.RNA_SEQ],
)

Error:

<_MultiThreadedRendezvous of RPC that terminated with:
	status = StatusCode.PERMISSION_DENIED
	details = "Received http2 header with status: 403"
	debug_error_string = "UNKNOWN:Error received from peer  {created_time:"2025-07-16T14:58:26.142485828+00:00", grpc_status:7, grpc_message:"Received http2 header with status: 403"}"
>

Is my API key misconfigured? Is this likely some other environment issue? I’ve tried restarting my kernel. Are there other debugging steps I could take?

Thanks,
Jane

2

Hi @Jane_Brown, welcome to the forum!

Does the snippet above work when run locally in python env, or via https://colab.research.google.com/ with your API key? if so that’d suggest it’s a connection issue from All of Us to our servers.

3

Looks like it works fine on Colab. It must be an issue with the All of Us platform. I’ll follow up with their support team. Thanks so much!