Shifted gene annotations in mm10

Hi, I’m currently predicting RNAseq tracks in mouse mm10 - I’ve centred my region on my gene of interest, and my plot shows transcription peaks at the expected locations looking at the scale markings at the bottom of the plot, but the gene locations from the gtf files are in the wrong coordinates. It looks like there is an error in how the gene transcripts have been plotted in the top line - has anyone else had a similar experience to this, or suggestions on how to fix it?

Hi There!

Thanks for reaching out.

May you please share a screenshot showing the apparent annotation misalignment, as well as the genome coordinates?

Kind regards,
Tumi

Thanks Tumi!
These 2 peaks in the plot below should be directly under the genes Hba-a1 and Hba-a2, coordinates mm10:
chr11:32,283,672-32284,486 # hba-a1-gene
chr11:32,296,492-32,297,310 # hba-a2-gene