Question about the difference between RNA-SEQ Quantile Score and predict_variant visualization

Help & Support
1

Hello,

I used the score_variant function to calculate the score of a variant of interest. Based on RNA-SEQ, the results were as follows: quantile_score = 0.999079 (raw = 0.014495). (The analysis was performed at the gene level.)

However, when I visualized the same variant using predict_variant according to the manual, I obtained a result where the REF expression was higher than the ALT expression. The predict_variant analysis was also performed using RNA-SEQ as the basis.

I would like to clarify whether I have misunderstood the meaning of the quantile_score, or if there may have been an issue with the visualization method.

포럼 지룸
포럼 지룸1855×401 62.5 KB

Thank you in advance for your response.

2

Thank you for the message. Could you provide an example interval and variant where you see this issue.